Enzyme Cleaning Products

What Actually Makes Pet Urine Smell, and Why Everyone Says Uric Acid

By ECP Staff · Published August 19, 2026

Fresh urine barely smells. What you smell a day later is the deposit it left being worked on by bacteria already in the carpet: urea split into ammonia by bacterial urease, and in cats a second sulfur pathway on top of it. That is why 'it smelled fine for a week' is normal rather than a failure, and why killing bacteria with a disinfectant pauses the smell without removing what feeds them. The enzyme method removes the deposit instead, which is the only step that compounds.

What to buy for this: Simple Solution Extreme Stain and Odor Remover 1 gallon (128 fl oz) · $37.49 · the cheapest for its size that we track in this category

Two molecules make the smell, one of them only in cats, and neither one is the molecule the whole category blames.

What Pet Urine Is Made Of, and Which Part Smells

Fresh urine barely smells. What you smell a day later is the deposit it left, being worked on by bacteria already in the carpet.

Urea:
The compound carrying most of the nitrogen in urine. Bacteria split it into ammonia, and that is the sharp note.
Urease:
The bacterial enzyme that does the splitting, and why a fresh puddle smells mild and the same spot tomorrow does not.
Felinine:
A compound only cats put in their urine, odorless when it lands.
Salts:
What is left when the water evaporates. Salts pull water back out of humid air and restart the bacteria.
Not uric acid:
Odorless, and both animals converted it before the urine left the body.

The delay is the tell. A puddle that lands nearly odorless and ripens over a day is being worked on by organisms that arrived with it, and every treatment decision follows from that delay.

Composition sets the removal logic. Each fraction responds to a different tool, and the fractions live together in one deposit, which is why the successful products are broad rather than targeted.

The bacteria are resident rather than introduced. They come in on the soil, sit dormant while everything is dry, and wake every time the deposit rewets, which the salts arrange on a schedule the weather keeps.

Salts are the memory of the accident. They hold the residue in a form that waits, and waiting is what distinguishes a deposit from a stain: a stain shows, a deposit produces. The smell belongs entirely to the producing side.

Nothing in the list requires uric acid, and the list is complete without it. The ammonia pathway and the salts between them account for the dog smell, and the cat adds its own sulfur track on top.

Specifications for What Actually Makes Pet Urine Smell, and Why Everyone Says Uric Acid
What smells in dog urine Ammonia, made by ordinary bacteria splitting the urea in the deposit
What smells in cat urine The same ammonia, plus a sulfur compound cats make and dogs do not
How long the cat smell takes to appear 12 to 24 hours after the accident
What does not smell Uric acid.
Why it returns in warm weather The salt fraction pulls water back out of humid air, at roughly 55 to 75 percent relative humidity
What an enzyme or bacterial cleaner removes The organic deposit that keeps regenerating both odors
What it does not remove The odor molecules. No cleaning enzyme has a bond to cut in either one
Dwell that makes the difference 4 to 24 hours kept wet on carpet, per Bridgepoint's trade instruction sheet

Why Cat Pee Smells So Much Worse Than Dog Pee

Cats add a second smell that dogs do not have, and it is the one people recognize across a room.

Cats put an enzyme called cauxin into their urine, and cauxin releases a compound called felinine. Over roughly 12 to 24 hours that breaks down into a sulfur compound, 3-mercapto-3-methylbutanol, which Miyazaki and colleagues call the primary contributor to the odor of cat urine.

Yesterday is worse than this morning:
The smell you are chasing was built overnight.
Intact males are worst:
Felinine output is testosterone-regulated.
Dog products are not undersized cat products:
Dog urine is largely the ammonia note. Cat urine is that plus sulfur.

The sulfur track runs on its own clock. A compound that builds over the first day means the worst smell postdates the accident, and treatment sized to the morning smell meets a stronger deposit by evening.

Time also works against the search. The room that read mild on accident day reads heavy a day later, and mapping done too early undershoots the area that will eventually fluoresce and smell.

Recognition across a room is a concentration effect. The cat compound carries at lower concentrations than the ammonia note, which is why a house can pass a floor-level sniff and still announce itself at the door.

Testosterone moving the output moves the treatment target. An intact male's deposits carry more of the precursor. The same physical treatment has to be more thorough to reach the same result, which is a volume statement rather than a product one.

The dog comparison is a chemistry comparison, not a difficulty contest. Both deposits respond to the same method, and the cat's extra fraction only raises the standard of execution: wider, wetter, longer.

Why the Urine Smell Comes Back in Humid Weather

Because the spot never dried in the sense you meant. Only the water left.

Everything dissolved in the urine stayed behind as a salt deposit in the fiber, the backing and the pad. Salts take water back out of humid air, which rewets the deposit and restarts the bacteria. Cleanfax puts the trade version in one line: alkaline salts are hygroscopic, meaning they absorb moisture from the air.

One correction we owe, because we published the wrong version ourselves. The recrystallizing-uric-acid story has no primary support, and no published deliquescence point exists for uric acid or urate.

The loop runs on air moisture alone. The salts pull water from the room, the water mobilizes the residue, the bacteria resume, and the smell returns without any new accident to account for it.

Seasonal return is therefore diagnostic. A smell that tracks the weather rather than behavior was never removed, however quiet the winter was, and the calendar is the instrument that reads it.

Dry treatment days and humid verdict days are different regimes. A treatment judged in dry cold air graded a paused deposit, and the same treatment judged in warm humidity grades the deposit itself.

The correction about uric acid matters here because the replacement mechanism has a different feel. Salt rehydration is gradual and reversible, which matches the smell that fades and returns, rather than a crystal dissolving once and being done.

Controlling room humidity controls the symptom without touching the cause. A dehumidified basement can read clean for years while holding the same deposit, and the first humid week re-pronounces the original verdict.

What we believe
The smell returns because the organic and salt deposit is still in the assembly, not because the odor molecules survived a treatment.
What would change our mind
A measurement showing a spot with no remaining urea or organic deposit that still regenerates ammonia on a humid day.
What we could not verify
Any published critical relative humidity for uric acid or urate. None exists, which is why we describe the salts instead.

Why Almost Every Article Blames Uric Acid

Because the publishers have been copying each other, and the story is tidy. It is wrong in two separate places.

Uric acid is odorless:
PubChem lists it as an odorless white solid. A molecule with no smell cannot be the smell.
Cats and dogs barely excrete it:
They carry uricase in the liver, which converts uric acid to allantoin, and allantoin is what leaves in the urine.
The exception is narrow:
Dalmatians and dogs with a portosystemic shunt do excrete high urate, which Bannasch and colleagues mapped in PLoS Genetics.

Of 24 competitor pages we examined, eleven assert uric acid and none mentions allantoin.

The story sticks because it is tidy. A crystal that survives cleaning and re-dissolves in humidity explains everything a reader wants explained, and a publisher has no commercial reason to check it against a metabolic pathway.

Copying is cheaper than checking. The claim needed one confident page, and every later page could cite the one before it, which is how an error becomes a consensus without ever becoming a fact.

The metabolic fact is not obscure. The pathway that converts uric acid before it leaves the body is standard physiology in both animals. The compound everyone blames is mostly not in the bottle it is blamed on.

The exceptions prove the pathway rather than the rule. Where the conversion fails, the compound shows up, and the population with that failure is narrow enough to name, which is the opposite of a universal claim.

Getting the molecule right matters for expectations. A reader who believes an insoluble crystal is at fault buys products aimed at it, and the honest page owes them the two molecules that are actually present.

The count of pages carrying the error is a snapshot rather than a feud. It is the error rate of a copy culture, and the number exists to be rechecked, not defended.

What an Enzyme Cleaner Actually Does to Urine

It takes apart the deposit that keeps making the smell. It does not attack the smell.

Protease:
Cuts protein, which covers the protein fraction of urine along with blood, sweat and milk.
Amylase:
Cuts starch, which is very little of what is in urine.
Lipase:
Cuts fats and oils, including the lipids urine leaves in carpet.
Uricase:
The enzyme that would consume urate. No consumer product we read contains it.

Each is a hydrolase, defined by the one bond it cuts, and there is no bond for one to cut in ammonia or in the cat sulfur compound. Remove the organic deposit and the bacteria lose their food.

Cutting is mechanical rather than magical. Each enzyme class holds one shape and cuts one bond. The deposit comes apart only where those bonds exist, which is protein, starch and fat rather than the small smell molecules themselves.

Removal is the consequence of size. Big molecules anchored in fiber become small ones water can carry, and the rinse or the extraction takes them the rest of the way out of the assembly.

The bacterial route to the same end runs through appetite. A bacterial product grows a population on the deposit. The population secretes its own cutting tools on site, which is slower to start and longer-lived than a dose of free enzyme.

Removing the organic fraction removes the smell's economy. The ammonia pathway runs on urea worked by resident bacteria, and the cat compound runs on its own precursor. Both precursors are part of the deposit rather than part of the air.

The honest limit is the one already named. No consumer product carries the enzyme that would consume urate, and the mechanism that does work needs no inflation to justify it.

Why It Smells Worse Right After You Clean It

Because you rewet a dried deposit and released everything in it before anything degraded it. Hour one smells worse than hour zero, and that spike means the liquid reached the contamination.

The counter-evidence belongs here rather than in a footnote. Bacillus species produce urease, and urease liberates ammonia from urea. No published net-ammonia balance exists for a bacterial product on treated carpet, so we cannot size that effect.

The sequence that resolves it is dwell, then remove.

The spike is release before reduction. Liquid entering a dried deposit dissolves and mobilizes what it has not yet cut, and the first hours are the deposit's most available hours.

Read the spike as a depth report. A treatment that smells strongly at hour one touched a large reservoir, and the size of the early note scales with what the pour reached. No spike at all suggests the liquid never met the deposit.

The bacterial contribution runs both directions. The organisms that make ammonia from urea are the same kind of organism a bacterial product seeds. The net direction of the ammonia balance is a measurement nobody has published for a treated carpet.

Uncertainty about the balance does not change the instruction. Dwell and remove remains the sequence that resolves both mechanisms, because time is what the cutting needs and extraction is what stops the mobilized residue from settling back.

Judging inside the spike window mistakes process for failure. The treatment is working most visibly at exactly the hour a first-time user is most likely to blot, and the schedule exists to keep those two events apart.

How to Remove a Urine Smell Instead of Pausing It

On an absorbent assembly, meaning carpet, pad, tack strip and subfloor. Hard floors and moisture-sensitive surfaces need a different method.

The method is a sequence, and each step exists because the step before it leaves specific conditions behind. Finding sets the area, blotting removes what is loose, the margin covers the geometry, and the pour only has meaning once those are settled.

Blotting before pouring protects the dose. Standing liquid dilutes what you add, and a towel under weight pulls the loose fraction out so the treatment lands on deposit rather than on wastewater.

The margin exists because the deposit is wider than the mark. Under the carpet the liquid spread sideways, and the tape marks the boundary of that spread as well as it can be seen from above. Treating to the tape alone treats the average of the deposit rather than its edge.

Covering is dwell insurance. The reaction runs on wet contact, and an open pour in a warm room dries long before the window closes. The sheet converts hours of air exposure into hours of work.

The fan at the end belongs to removal, not to the treatment. Drying after the dwell carries off the water and what it dissolved, and no heat is wanted because heat costs the biology more than it costs the water.

Judging warm and low is judging honestly. The deposit's signature returns with warmth and moisture, and the nose belongs at floor level where the heavier volatiles collect. A verdict taken standing, in a cold room, is a verdict taken under the wrong conditions.

  1. Find the whole spot with a longwave ultraviolet light in a dark room, then mark the outer edge with painter's tape.

    1 dark room

  2. Blot the marked area with a folded white towel, standing on it for 30 seconds per pass, until the towel comes up close to dry.

    30 seconds

  3. Extend the treatment area 6 inches past every taped edge.

    6 inches

  4. Pour it, do not mist it, and keep pouring until the carpet is wet across that whole extended area and the pad underneath is wet too.

    1 pour, not a mist

  5. Cover the wet area with a plastic sheet cut 12 inches wider on every side.

    12 inches

  6. Leave it 4 to 24 hours, which is the dwell Bridgepoint specifies for its trade bacterial additive.

    4 to 24 hours

  7. Pull the plastic and dry the area with a fan and no heat.

    0 added heat

  8. Judge it at 48 hours, at floor level, in a warm room.

    48 hours

Why an Enzyme Cleaner Has to Stay Wet on a Urine Spot

Keeping it wet. Not pouring more, not buying a better bottle: covering the spot so it cannot dry.

Evaporation ends the reaction and the clock has nothing to do with it. Germination takes minutes, outgrowth takes one to two hours by live-cell imaging, and secretion starts after that. A ten-minute blot ends the job three steps before the first enzyme exists.

Cleanfax states the field version and its limit together. The area has to be kept moist and warm for 24 to 48 hours, which is difficult to sustain on carpet in the field.

The timeline stacks. Germination, outgrowth and secretion arrive in that order, and none of them run in dry fiber. A spot that dries at any stage stops at that stage, and a ten-minute blot stops everything before the first tool exists.

Evaporation is the terminator the label never names. The bottle states hours, the room enforces evaporation, and the gap between the two is the cover's job to close. On that accounting, the plastic is part of the dose.

Wet contact is also transport contact. The pieces the enzymes cut need water to move away from the fiber, and a drying assembly strands them where they formed. Wet is not merely the working condition, it is the removal condition.

The trade's own difficulty note sets expectations. Field conditions fight a long moist dwell on carpet. That is why the cover is weighted, the room is closed, and the hours are counted from wet, not from the pour.

Nothing about the product's quality changes this arithmetic. An excellent bottle blotted dry at ten minutes underperforms an ordinary one held wet overnight, and the difference is entirely in the conditions the user controlled.

How to Tell the Urine Smell Is Gone and Not Just Dormant

Reproduce the condition that brings it back. Sniffing a cold dry spot in February tells you nothing.

The warm damp test:
Wait 48 hours after the area is fully dry. Lay a towel wrung out in warm water over the spot for 20 minutes, then smell at floor level.
The ultraviolet recheck:
Residual glow tells you where to pour next time. Blue usually means optical brightener from an earlier cleaning attempt.
The season test:
The real verdict arrives on the first humid day.

Each test manufactures the conditions of return. Warmth, moisture and time are the levers that reactivate a dormant deposit, and every test on the list pulls at least one of them on purpose.

The towel-and-warm-water pass is the humid day in miniature. It delivers moisture to the salts and warmth to the biology, the interval is long enough for the exchange, and the floor-level nose is positioned where the answer arrives.

The recheck under light reads residue rather than smell. Glow marks where deposit remains, and the pattern after treatment tells the next pour where to go, which is more useful than a bare verdict.

Seasonal patience is the final court. A deposit can hold quiet through a dry stretch and answer on the first humid afternoon. The reader who knows that watches the weather rather than the calendar of treatments.

A pass is provisional in one direction only. Passing every test strongly suggests removal, and a quiet week proves nothing beyond quiet. The tests are designed to catch the false pass, and they are worth running exactly as long as the spot has history.

Why the Urine Smell Came Back After You Cleaned

Six causes, and the bottle is the last one to suspect.

A return has a short list of possible authors, and the branches are that list. Delivery failures, dwell failures, prior chemistry, depth and re-soiling each leave a different signature, and reading the signature is faster than re-running treatments at random.

The treatment can have succeeded completely and the animal re-established the spot within days, and the smell that follows is new deposit rather than old residue.

Separating those two takes one question: did the smell return on a schedule, or with the weather. Weather implicates residue, behavior implicates a new deposit, and the two answers route to different work entirely.

The depth branch earns its place at the end of the list. It is the only cause a bottle cannot address, and reaching it means every correctable cause was closed first, which is what three soakings actually accomplish.

Match before you treat. Every branch except depth has a specific corrective, and running correctives in the wrong order burns the evidence that would have matched the right one.

Two rounds of reading are normal before the answer is clean. The first read assigns a cause, the second confirms it, and the smell that survives both is the one that has earned a structural look.

  1. The smell came straight back once the spot dried, or it returns on every humid day.

    Why: The liquid never reached the depth the urine reached.

    What to do: Re-treat at volume. Pour until the carpet is wet 6 inches past every edge of the mark and the pad below is wet.

  2. You followed the label, blotted the area dry after ten minutes, and nothing changed.

    Why: The product was removed before any enzyme existed. Spores germinate in minutes and take one to two hours to grow out.

    What to do: Re-treat, cover the area with plastic, and leave it 4 to 24 hours.

  3. The smell got worse in the first few hours after treatment.

    Why: You rewet a dried deposit and released the odorants before anything degraded them. That is evidence the liquid reached the contamination.

    What to do: Finish the dwell, then dry and extract rather than letting it evaporate in place. Judge it at 48 hours, not at hour one.

  4. You used a carpet machine, an oxygen cleaner or a peroxide spray on the spot first.

    Why: Oxidizers are the real risk to these products, and hydrogen peroxide kills a live bacterial product outright.

    What to do: Rinse the area with clear cool water, blot it, let it dry, then treat.

What Not to Put on a Urine Smell

Bleach is the one that can hurt you:
Hypochlorite meeting the ammonia in urine produces chloramine gas. America's poison centers logged 75,186 chlorine and chloramine exposures between 2015 and 2022.
Peroxide and oxygen cleaners are the real threat to the product:
The patent literature is blunt: hydrogen peroxide would kill the probiotics.
Ammonia belongs nowhere near it:
Ohio State's Indoor Pet Initiative tells owners never to use it on carpet, because the smell can draw the cat back.
Heat, until the removal step:
Rocco and Roxie says high heat deactivates its own biological ingredients.
A mist, in a house with asthma:
EPA's Safer Choice program will not certify a spray-delivered enzyme product.

The list ranks by who gets hurt. Bleach on ammonia-bearing deposit hurts the occupant, oxidizers hurt the product, ammonia hurts the training, heat hurts the biology, and mist hurts the susceptible airway. Each prohibition protects a different party.

Safety sits above efficacy for that reason. A failed treatment costs a bottle, and a chloramine exposure costs an emergency visit, and no cleaning outcome justifies trading the second for the first.

The oxidizer ban is about sequence rather than malice. Peroxide belongs in the cupboard for other jobs, and its crime here is residue: an antimicrobial film in the fiber that a bacterial treatment lands on and dies in.

Heat reads as help and works as sabotage. Warmth from a machine arrives with temperatures the biology does not survive. The same pour that would have worked on Tuesday meets a deactivated workforce after a Sunday steam pass.

The mist warning is conditional and stands anyway. Pouring is the method on carpet regardless, and in a house with asthma the applicator is the decision, which is why the guidance names it rather than the product.

Whether to Keep Treating the Spot or Replace What Holds It

After three proper soakings:
Poured to volume, covered, left 4 to 24 hours. If it survives that, it is in the pad, the tack strip or the subfloor. **When the animal is telling you something.** A cat that used the box and stopped needs a vet, not a cleaning strategy. **When the boards are black.** Dark staining in wood is a tannin reaction under the finish, and that is a sanding decision. **When it is in the ducts.** A floor register under a repeat spot moves the smell through the whole house. Cleanfax gives the rule: the cost of odor control should not exceed the cost of replacing the textile.

Each stop condition names the evidence that triggers it. Three soakings is a count, the vet line is a behavior change, black boards are a visible reaction, and the duct is an airflow pattern. All four are observable before the money moves.

The soaking count is the only condition a reader controls fully. Depth, tannin and duct are facts the house supplies, and the threshold exists to stop spending against facts rather than to encourage it.

Replacement decisions are surface decisions. The comparison the trade rule prices is textile against effort, and the reader's version prices pad against bottles, which is the same arithmetic at a different scale.

The vet line outranks everything else here. A medical cause produces accidents at a rate no cleaning schedule can match, and treating the symptom while the cause runs is the most expensive sequence available.

$11.89 to $59.97 Typical spend on this job

Prices read from our own catalog on August 19, 2026.

What We Could Not Verify on This Page

Three gaps, named. No published critical relative humidity exists for uric acid or urate, so the humidity section describes the salts. No published net-ammonia balance exists for a bacterial product on treated carpet, so the urease objection stays unsized. No manufacturer or trade source publishes a volume of cleaner per volume of urine, so we publish the principle and no multiplier.

The three gaps share a shape: each sits where a measurement would settle an argument, and none of them sits where a reader waits on an answer. The method runs on published mechanism, and the gaps bound the claims rather than the instructions.

The humidity gap is why the salts carry the explanation. Without a published reactivation point for the blamed compounds, the honest text explains the water-handling behavior that is documented and lets the reader watch the weather rather than a number.

The ammonia balance gap keeps the urease objection alive rather than resolved. Bacillus can add ammonia while it removes the deposit, and nobody has netted the two on a real carpet, so the objection rides along instead of a verdict on it.

The volume gap returns the decision to geometry. No multiplier exists to quote, so the instruction is edge, margin and wetness, and a reader with a tape and a towel can execute it without the missing figure.

Absence is stated plainly because invention is the industry default here. Every one of these gaps has been filled by somebody's marketing at some point, and the fill was always a number with no source behind it.

Claim Source Tier
Cauxin releases felinine in cat urine, and felinine degrades to 3-mercapto-3-methylbutanol, the primary contributor to the odor of cat urine cell.com Direct or original source
Dogs and cats carry hepatic uricase and excrete allantoin; Dalmatians are a urate transport exception pmc.ncbi.nlm.nih.gov Direct or original source
Uric acid is an odorless white solid with a water solubility of about 0.06 milligrams per milliliter pubchem.ncbi.nlm.nih.gov Direct or original source
Allantoin, the actual mammalian purine end product, is far more soluble than uric acid pubchem.ncbi.nlm.nih.gov Direct or original source
Of 24 competitor pages we examined for the uric acid claim, eleven assert it and none mentions allantoin enzymecleaningproducts.com Direct or original source
Bacillus spores germinate within minutes and take one to two hours to grow out before meaningful enzyme secretion journals.plos.org Direct or original source
Bio-Charge specifies a four to 24 hour dwell, instructs covering the treated area with plastic so the solution cannot dry, and is ready-to-use pH 7 spec-sheets.interlinksupply.com Direct or original source
Bridgepoint's own directions state that the area of contamination under the carpet may be more than twice the size of the stain on the surface spec-sheets.interlinksupply.com Direct or original source
ProRestore ODORx Un-Duz-It Unleashed is ready-to-use pH 9.0 with a one hour dwell and mandatory extraction specs.safetyexpress.com Direct or original source
Rocco and Roxie describes a consortium of non-pathogenic Bacillus spore microorganisms at one billion colony-forming units per gram, says deep set-in urine wants a couple of days, and warns that high heat deactivates its biological ingredients roccoandroxie.com Direct or original source
Nature's Miracle safety data sheet gives product pH 6.25 to 7.5 and withholds the composition as a trade secret assets.unilogcorp.com Direct or original source
Alkaline salts are hygroscopic and absorb moisture from the air; the area must be kept moist and warm for 24 to 48 hours, which is difficult to sustain in the field; the cost of odor control should not exceed the cost of replacing the textile cleanfax.com Secondary source
The trade volume rule is to recreate the crime: add as much deodorizer as there is contamination present in the carpet and pad cleanfax.com Secondary source
Hydrogen peroxide is a natural antimicrobial and would kill the probiotics in a live bacterial formulation patents.google.com Direct or original source
An opposing patent family treats carpet urine odor with urease deliberately, which is the strongest technical objection to bacterial products patents.google.com Direct or original source
Subtilisin proteases are inactivated below pH 4 heraproject.com Direct or original source
Never use ammonia or ammonia-based products on carpet, because the smell can attract the cat back; Ohio State separately recommends white vinegar for laundry and linoleum indoorpet.osu.edu Secondary source
US poison centers logged 75,186 chlorine and chloramine exposures between 2015 and 2022, with household acid meeting hypochlorite causing 48.9 percent of isolated chlorine gas cases pmc.ncbi.nlm.nih.gov Direct or original source
Counted from the published product directory, prices read August 19, 2026: 3 pet products in a 32 ounce size at $12.72 to $23.97, 1 in a 128 ounce size at $37.49 enzymecleaningproducts.com Direct or original source
Dried urine leaves an alkaline salt residue and those salts absorb moisture from the air, which rewets the deposit and restarts the bacteria. The 55 to 75 percent band is our own practical reading; no critical relative humidity is published for urate. The author of the cited piece sells an enzyme product line cleanfax.com Secondary source

Frequently Asked

Does enzyme cleaner remove urine smell?

Yes, when enough of it reaches the deposit and stays wet for hours. It removes the organic deposit that keeps making the smell rather than the odor molecules. Wetness is the condition that keeps the reaction running, which is why the same spot changes with the weather. The deposit does not need a new accident to smell again, only damp air and warmth. Removing it, rather than treating the air above it, is the only version that holds past the week.

What is the most powerful urine odor eliminator?

Power is the wrong axis and delivery decides it. Bridgepoint's Bio-Charge is stronger chemistry than anything on a supermarket shelf, and it still specifies a four to 24 hour dwell kept wet. Stronger chemistry also cannot beat wrong delivery. The trade concentrate still asks for hours of wet contact, because that is what the cutting reaction needs. A weaker product poured properly and covered outruns a strong one misted onto the surface for a minute.

Is there an enzymatic cleaner that can remove urine smells from bathrooms?

Yes, and the target is the grout and the floor around the base of the toilet rather than the porcelain. Grout is porous and holds the deposit where a wipe cannot reach. That is why bathroom smells survive months of mopping. The enzyme pour has to sit in the gap long enough to work, and repeat treatments are the norm rather than a failure. Find every gap the urine traveled into, because the base of the toilet hides more than the seat does.

More on the same problem in Pet Stains.